Cutting Edge Science & Technology

We have been using the digital system to detect signals from our chemiluminescent Western blots previously, however the old system sometimes struggled to cool the camera down to the required temperatures during hot summer months. This was especially a problem when we needed long exposure times to detect low abundance signals in our samples. Therefore, we decided to replace it with the newer and more reliable system. G:BOX Chemi XX6 imaging system from Syngene was the most sensitive device from all devices that we had a chance to test, including our own old system. Therefore, we decided to purchase the G:BOX Chemi XX6 with the upgrade for fluorescent applications, and since then we successfully use it to acquire chemiluminescent Western blots, protein gels with proteins in-house labelled with various fluorescent dyes, and occasionally, we also analyse and count the bacterial colonies expressing fluorescent proteins. We are happy we decided for this versatile and easy to use system.

Our imaging work is mainly chemiluminescent blots of proteins associated with inflammatory diseases and is done by PhD and post-doctoral scientists, as well as students on our Masters’ course in Applied Biosciences. We were using ECL/X-ray film for this, but it was costly, required a darkroom and was difficult to obtain good quantitative results, which is why we assessed automated imagers.

We decided to purchase a G:BOX Chemi XRQ because the imaging box is easy to upgrade and Syngene staff are more knowledgeable when we ask technical questions. Our G:BOX Chemi XRQ is now in regular use because it is simple for everyone to set up and produces good quality images. We are pleased we chose the G:BOX Chemi XRQ imager for our lab.